Brilliant II SYBR Green qRT-PCR, AffinityScript Master Mix, 2-Step Protocol. Account & Lists Returns & Orders. The Master Mix simplifies the assembly of PCR reactions and offers advantages of time savings, consistency, and minimal risk of contamination. qPCR 1-Step RT-qPCR 2-Step RT-qPCR. Figure 2. Brilliant III Ultra-Fast SYBR Green QRT-PCR Master Mix 1 Brilliant III Ultra-Fast SYBR Green QRT-PCR Master Mix MATERIALS PROVIDED Catalog #600886 (single kit), #600887 (10-pack kit) Materials Provided Quantity a,b 2 Brilliant III Ultra-Fast SYBR Green QRT-PCR Master Mix 2 2 ml RT/RNase Block 400 l 100 mM DTT 100 l * The improved Brilliant II formulation yields higher levels of final fluorescence and earlier Ct values for many genomic DNA and cDNA targets and the master mix format is ideal for high-throughput applications. Sahara One-Step RT-qPCR Master Mix with UNG is a high performance mix for multiplex, probe-based quantitativie reverse transcription PCR (RT-qPCR). Agilent Brilliant II QPCR Master Mix; High performance, ultra sensitive, next generation QPCR master mix reagent for reliable quantification across a wide range of targets and templates. On the Experiment Properties screen, select TaqMan Reagents and the Fast ramp speed. Agilent technologies brilliant iii ultra fast sybr green qpcr master mix Brilliant Iii Ultra Fast Sybr Green Qpcr Master Mix, supplied by Agilent technologies, used in various techniques. Numerous enhancers bind & inactivate PCR inhibitors, allowing amplification from crude . 400 rxns Get Quote RUO Brilliant III ultrafast SYBR qRT-PCR Master Mixes provide specificity by utilizing a novel faster-activating hot start method. Brilliant II qPCR Master Mix Catalog Code: 600804 Product Unit: 400 reactions List Price: $593.00 Special Price: $415.10 Save: 30%. ZERO BIAS - scores, article reviews, protocol conditions and more Log In or Create a Profile. 4 Brilliant SYBR Green QPCR Master Mix Figure 1 SYBR Green I dye has a higher affinity for double-stranded DNA (dsDNA) than for single-stranded DNA or RNA. Obtain predesigned assays for human, mouse, or rat for easy selection based on multiple criteria such as exon location and number of transcripts detected. It quantitatively amplifies singleplex and duplex qPCR targets at high efficiency from purified samples and crude extracts. In the amplification plot (top panel) the reactions containing template show a Size. The product range allows you to more successfully multiplex without concern for bias due to abundance level. Brilliant II qRT-PCR Core Reagent Kit, 1-Step Protocol. formulation that includes all components for qPCR except sample DNA, primers and water. Once thawed, full activity is guaranteed for 6 months. MCE qPCR Master Mix can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability. Get Quote from Agilent Technologies for Brilliant III Ultra-Fast SYBR Green QPCR Master Mix, 10 pack. Brilliant III qPCR probe master mix with ROX is one of our fastest, high-performance, ultrasensitive, next generation qPCR master mix reagents. Available in two formulations, one with ROX . Master mix degradation is a leading cause of inconsistent C q values in PCR. Visit this page to find out more. The mix supports both standard and fast protocols, and amplifies GC-rich targets up to 70%. Extremely fast while maintaining sensitivityGreater resistance to common QPCR inhibitors (whole blood or NaCl)Optimized fast cycling formulation ensures reliable and reproducible data . QPCR and QRT-PCR master mixes allows you to use cell lysates directly in a real-time QPCR amplification - skipping RNA purification steps. Hello, Sign in. License-free for commercial or diagnostic use Description Chai Green qPCR Master Mix is a 2X Taq mix for dye-based Real-Time PCR, melt curve analysis, and High Resolution Melt (HRM). Compare this item. Perfect for researchers who want their data faster without compromising . Price from $9.99 to $1999.99 brilliant ii sybr green qpcr master mix - by Bioz Stars , 2022-08 86 / 100 stars Images 1) Product Images from "Effects of sodium chloride on heat resistance, oxidative susceptibility, motility, biofilm and plaque formation of Burkholderia pseudomallei. 5ml 25ml. qPCR was performed in an Mx3000P qPCR System (Stratagene) using Brilliant SYBR Green qPCR Master Mix (Stratagene) according to the manufacturer's instructions. The single-step master mix format is ideal for most high-throughput QPCR applications where it is not necessary to archive cDNA. $ 340.00. Description. Brilliant III Ultra Fast SYBR qRT-PCR Master Mixes provide specificity by utilizing a novel faster-activating hot start method. Brilliant III Ultra-Fast SYBR QPCR and QRT-PCR Master Mixes deliver superior specificity by utilizing a novel faster-activating hot start method to minimize the formation of primer-dimers and other off-target reactions. Bioz Stars score: 98/100, based on 1 PubMed citations. Designed for fast, highly- sensitive real-time RT-PCR even in the presence of challenging PCR inhibitors. Fisher Scientific ; Fisher Healthcare ; Fisher Science Education ; Sign Up for Email NEW Help & Support Center +1-800-766-7000; 0. Brilliant II SYBR Green QPCR master mix includes the components necessary to carry out QPCR amplifications with SYBR Green detection. Amplify up to four targets in a single real-time PCR Reaction, The Brilliant Multiplex QPCR Master Mix provides sufficient reaction components to accurately quantify both low-and-high- . Brilliant II SYBR Green qRT-PCR Master Mix Kit 1-Step Protocol. On the Run Method screen, set the reaction volume to 20 l and adjust Compatible with both fast and standard cycling methods. Features Fast kit with speeds at 40 min per 40 cycles Kit comes in master mix format and includes hotstart enzyme Low-abundance RNA, down to 10 fg Amplification and detection from total RNA, poly (A+), and synthetic RNA samples Multiplexing capability Reagents validated across a range of fast real-time PCR cyclers Return to top Specifications This method prevents sample loss and degradation, ensuring accurate gene quantification in downstream QRT-PCR. Application Kits ng S Library Quantification Optimal cluster density improving efficiency and data quality MycoSensor Detects common mycoplasma strains, down to 50 copies gDNA The kits include novel, fast Taq mutant for qPCR results in less than 35 minutes. Brilliant II qPCR Master Mix Catalog Code: 600815 Product Unit: 10 x 400 reactions List Price: Special Price: Save: 30%. Brilliant kits support quantitative amplification and detection. These reagents are robust and reproducible across many assays on fast cycling real-time platforms. Upon binding dsDNA, the fluorescence yield of SYBR Green I increases by approximately 1000 fold, making it ideal for detecting the accumulation of dsDNA. Agilent Brilliant II QPCR Master Mix, 600815 Life Science Molecular Biology Kits PCR-related Kits Agilent Brilliant II QPCR Master Mix, 600815 Share this : Print Agilent Brilliant II QPCR Master Mix, 600815 Agilent Brilliant II QPCR Master Mix - SR (Additional S&H or Hazmat Fees May Apply) List Price $4,442.00 Your Price $4,442.00 These reagents are robust and reproducible across many assays on fast cycling real-time platforms. Brilliant II qPCR and qRT-PCR reagents maintain the high quality of our original Brilliant product line. It amplifies RNA targets at high efficiency while resisting PCR inhibitors. Ready-to-use mix of optimized components for dye-based qPCR or RT-qPCR. Expand View more product information Add to cart Send us an Enquiry Brilliant Multiplex qPCR Master Mix Brilliant II QPCR Low ROX Master Mix When large plate-based experiments can take over an hour just to set up, it can be hard to control for the effects of enzyme degradation. KAPA SYBR FAST qPCR Master Mix (2X) is designed for high-performance real-time PCR. Agilent technologies brilliant multiplex qpcr master mix solution Brilliant Multiplex Qpcr Master Mix Solution, supplied by Agilent technologies, used in various techniques. SYBR Green dye is a fluorescent double-stranded DNA (dsDNA)- binding dye that is used to track the progress of DNA amplification in real-time PCR experiments. Brilliant III QPCR and QRT-PCR master mixes increase overall sensitivity and speed through the novel, quick-activating hot start and the engineered Taq-mutant.The wide range of reagents are suitable for use with nucleic acids in transfection and transformation procedures, as well as cloning, sequencing, purification, and extraction. Log In or Create a Profile. The NovaTaq PCR Master Mix is a ready-to-use 2X mixture of NovaTaq DNA Polymerase, ultrapure deoxynucleotides, and reaction buffer without MgCl2. Supplier Page We Believe You Are Important, How Can We Help? General purpose reagent provides sensitive, reproducible detection even in the presence of challenging PCR inhibitors. ADDITIONAL MATERIALS REQUIRED Price from $9.99 to $1999.99 brilliant ii sybr green qpcr low rox master mix - by Bioz Stars , 2022-08 80 / 100 stars Buy from Supplier brilliant ii sybr green low rox qpcr master mix ( Agilent technologies ) 94 Agilent technologies brilliant ii sybr green low rox qpcr master mix Brilliant III ultrafast maintains amplification efficiency, R2, dynamic range, and detection sensitivity while shortening total cycle time by ~60% compared to standard cycling. Brilliant III QPCR and QRT-PCR master mixes increase overall sensitivity and speed through the novel, quick-activating hot start and the engineered Taq-mutant. Master mixes were run under recommended cycling conditions using 5 ng of cDNA from human total RNA in each 20 l reaction designed to detect the gene target using TaqMan 'Assays-on-Demand' primers/probe sets. E. . PrimeTime qPCR Probe Assays consist of a primer pair and fluorescently labeled 5 nuclease probe. SYBR Green qPCR Master Mixes; Brilliant II SYBR Green QRT-PCR AffinityScript Master Mix, 2-Step; . Dilutions of this cDNA were subsequently used for real-time PCR (cDNA equivalent to 6 ng of initial RNA per 15 L qPCR reaction for all genes). Comparison of robustness of Brilliant III Ultrafast qRT-PCR SYBR Green (blue bar) to company Qi qRT-PCR Master Mix (red bar) across 99 cDNA targets (10 ng each) on the ABI StepOnePlus Real-Time qPCR system. High performance, ultra sensitive, brighter and faster next generation QRT-PCR 2-step master mix reagent with AffinityScript RT for reliable quantification across a wide range of targets and templates. The Brilliant II qPCR SYBR master mix includes the components necessary to carry out PCR amplification in one tube and one buffer. High performance, ultra-sensitive, probe QPCR master mix reagent with high or low ROX concentration for reliable quantification across a wide range of targets and templates.The wide range of reagents are suitable for use with nucleic acids in transfection and transformation procedures, as well as cloning, sequencing, purification, and extraction. Note The SYBR Green I dye and the reference dye are light sensitive and should be kept away from light whenever possible. In Figure 1, the Brilliant III Ultra-Fast SYBR Green QPCR master mix was used in reactions containing serially diluted cDNA template (0.5 pg -50 ng) and a no-template control reaction (NTC) to amplify the GAPDH target. Brilliant SYBR Green QPCR Master Mix: Store all components at -20C upon receipt. The Brilliant multiplex QPCR master mix contains MgCl 2 at a concentration of 5.5 mM (in the 1 solution), which is suitable for most targets. SYBR Green Master Mix Advantages. Numerous enhancers bind & inactivate PCR inhibitors, allowing amplification from crude extracts of: Blood. No offer available; Chemical Structure . The one-step mix allows cDNA synthesis and subsequent PCR amplification to be performed in a single reaction . TaqMan Fast Virus 1-Step master mixes. 25 uL/reaction. It performs QRT-PCR in less time without compromising target detection sensitivity, specificity, or reproducibility. Food & beverages. Add to cart. Brilliant II SYBR Green High ROX QPCR Master Mix from Agilent Technologies Be the first to write a review! Minimizes the formation of primer-dimers and other off-target reactions. Brilliant Multiplex qPCR Master Mix Protocol. Brilliant III ultrafast maintains amplification efficiency, R2, dynamic range, and detection sensitivity. Probe Design Probes should have a melting temperature that is 7-10C higher than the annealing temperature of the primers. Minimizes the formation of primer-dimers and other off-target reactions. Amplifies Everything Without Inhibition. Resistant to a wide range of PCR inhibitors. 2 Complete the Setup screens for a new experiment as needed. Brilliant II QPCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations. 3/14/2016 Relative Quantification of Bacterial Gene Expression Dr. Thippeswamy Sannasiddappa Expression of Escherichia coli genes responsible for acid resistance were measured in relative terms using Agilent's brilliant ultra-fast SYBR green qPCR master mix in a 20 microliter reaction. Bioz Stars score: 80/100, based on 40 PubMed citations. Now has greater detection sensitivity, ensuring reproducible quantification even at low target concentrations. Shorter total cycle time. Sahara Multiplex qPCR Master Mix has been validated against genomic, plasmid, and cDNA targets from a wide range of organisms. Hot Start Polymerase, MgCl 2, dNTPs and a proprietary reaction buffer, produce optimal results in qPCR experiments. Extension Denaturing The Brilliant III Ultra-Fast QPCR low ROX master mix contains MgCl2at a concentration of 5.5 mM (in the 1 solution), which is suitable for most targets. As the PCR reaction proceeds, at each round of amplification SYBR Green dye binds to dsDNA as it polymerizes, resulting in an increase in the level of fluorescence at the end of each extension step. Get Quote. The Brilliant SYBR Green QPCR Master Mix is a single-tube reagent, ideal for most high-throughput QPCR applications. TaqPath 1-Step RT-qPCR Master Mix, CG. Brilliant III qPCR and QRT-PCRE Master Mixes are more sensitive and faster via the quick-activating hot start and the engineered Taq-mutant. The cycling conditions included an initial activation of the Taq DNA polymerase at 95C for 10 min, followed by 40 cycles of 15-s denaturation at 95C, and 1-min annealing . Brilliant Multiplex qPCR Master Mixes Amplify up to five targets in a single well with the sensitivity seen in single-plex qPCR reactions. We Believe You Are Important, How Can We Help? The mixes show consistent high performance with various SYBR detection systems. Agilent Brilliant III Ultrafast SYBR Green qRT-PCR Master Mix kits are more robust across multiple targets and can be used on non-fast cyclers. Alternatively, the combination of Stratagene's StrataScript First Strand cDNA Synthesis Kit (catalog #200420) and the Brilliant SYBR Green QPCR Master Mix (catalog #600548) provides RNA quantification in Reliability Comparison of Brilliant III QPCR Master Mix versus Company R Master Mix on 83 TaqMan 'Assays-On-Demand' Gene Targets. Better Melt Curves & HRM For faster experiments with earlier Ct's and greater reproducibility, Agilent recommends Brilliant III Ultra-Fast master mixes. The Brilliant SYBR Green QPCR master mix includes the components necessary to carry out PCR amplifications*, and has been successfully used to amplify and detect a variety of genomic and plasmid DNA targets. These unique reagents are equally robust and reproducible across a variety of assays on fast cycling real-time platforms. Importantly, the sensitivity remains equivalent to that seen in singleplex. Briefly, the required amount of template . SideStep cell lysates can be stored for up to 6 months before QRT-PCR analysis. The kit contains a novel DNA polymeraseengineered via a process of molecular evolutionresulting in a unique enzyme specifically designed for real-time quantitative PCR (qPCR) using SYBR Green I dye chemistry.
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