PrimeTime Gene Expression Master Mix performs well under standard or fast cycling conditions (Figures 4 and 5) on a variety of real-time qPCR instruments, including the 7900HT Real-Time (Thermo Fisher Scientific), QuantStudio 7 Flex (Thermo Fisher Scientific), CFX384 (BioRad), and LightCycler 480 (Roche) qPCR systems. Fast SYBR Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for your real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency. Use the real-time PCR master mix selection tool to find optimized master mixes, reagents, and kits to power your experiments. SuperScript IV VILO (SSIV VILO) Master Mix is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications. Search Thermo Fisher Scientific. Shop All Products. Alternative Product: Try TaqPath ProAmp Master Mix, our newest and highest performing master mix for genotyping and copy number variation for accurate results, even from samples containing PCR inhibitors.With TaqPath ProAmp Master Mix, weve taken the best of TaqMan Genotyping Master Mix and added additional capabilities for your genotyping and copy number variation Isothermal amplification. Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. Search Thermo Fisher Scientific. Inclusion of ezDNase Enzyme further accelerates the RT-qPCR workflow through an extremely simplified genomic DNA removal step. TaqMan Fast Advanced Master Mix is designed to provide performance equal to or better than the results you currently expect from your standard master mix. Get precise and reliable real-time qPCR quantification over nine logs of linear dynamic range with TaqMan Gene Expression Master Mix. TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. It provides best in class performance, including the widest quantifiable range, in both single and duplex reactions, even with challenging targets. Figure 2: The Luna Universal qPCR Master Mix provides sensitive and accurate detection and quantitation across a wide variety of DNA sources The Luna Universal qPCR Master Mix is compatible with a broad range of genomic DNA sources. Contains 1 X 1 mL tube, sufficient for 100, 20 L reactions (10 L Master Mix per reaction). TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. Probe qPCR MixTaqManDual Labeled ProbePCRqPCRPCRPCRPCR Genotyping & Genomic Profiling. FastReal-time PCR results in as fast as 35 minutes SensitiveDetect very low copies of target Search. Real-time PCR is the simultaneous amplification and detection/quantification of nucleic acids using PCR. Invitrogen SuperScript enzymes and reagents for superior cDNA synthesis. Search All and instruments for qPCR. In addition, TaqPath qPCR Master Mix, CG, has been engineered to retain consistent performance in preassembled reactions for up to 48 hours. qPCR is a versatile technique and a powerful tool to support your research applications. Key product features: High sensitivity4X master mix to amplify both RNA and DNA with improved sensitivity The stability of this mix provides users of high-throughput, liquid handling systems the assurance that the results on the first plate will mimic those of the last plate. Figure 2: The Luna Universal qPCR Master Mix provides sensitive and accurate detection and quantitation across a wide variety of DNA sources The Luna Universal qPCR Master Mix is compatible with a broad range of genomic DNA sources. Isothermal amplification. Alternative Product: Try TaqPath ProAmp Master Mix, our newest and highest performing master mix for genotyping and copy number variation for accurate results, even from samples containing PCR inhibitors.With TaqPath ProAmp Master Mix, weve taken the best of TaqMan Genotyping Master Mix and added additional capabilities for your genotyping and copy number variation Digital PCR products. The 2x mix contains SYBR GreenER dye, AmpliTaq DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific. TaqMan Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target. 1 tube TaqMan assay mix 1 tube master mix 1 tube quantitative genomic DNA standard 1 bottle DNA dilution buffer 1 tube negative control water. Shop All Products. Get superior nucleic acid quantitation with Applied Biosystems Power SYBR Green Master Mix.Power SYBR Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations. Search Thermo Fisher Scientific. Residual DNA Sample Preparation Kit contains: 1 box of buffers 1 box of magnetic beads 1 box of Proteinase K enzyme 1 box contains tRNA, Glycogen, Proteinase K Search Thermo Fisher Scientific. It provides best in class performance, including the widest quantifiable range, in both single and duplex reactions, even with challenging targets. Reverse transcription . qPCR is a versatile technique and a powerful tool to support your research applications. Get precise and reliable real-time qPCR quantification over nine logs of linear dynamic range with TaqMan Gene Expression Master Mix. 1 tube TaqMan assay mix 1 tube master mix 1 tube quantitative genomic DNA standard 1 bottle DNA dilution buffer 1 tube negative control water. TaqMan Fast Advanced Master Mix is designed to provide performance equal to or better than the results you currently expect from your standard master mix. (This master mix is also available without ezDNase.) Search All . Search Thermo Fisher Scientific. Genotyping & Genomic Profiling. Search All . TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The TaqMan Fast Virus 1-Step Master Mix provides a single-tube format for uniform handling and processing, while delivering qPCR cycling in <30 minutes, helpful when working in high throughput workflows. Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. In addition, TaqPath qPCR Master Mix, CG, has been engineered to retain consistent performance in preassembled reactions for up to 48 hours. Home. FastReal-time PCR results in as fast as 35 minutes SensitiveDetect very low copies of target PCR Equipment and Supplies. 50X higher fidelity compared to Taq; Robust reactions - maximal success with minimal optimization; Master mix format with HF buffer ideal for a wide range of AT/GC content RT-LAMP master mix for faster and simpler detection of RNA targets for viral research and surveillance. RT-LAMP master mix for faster and simpler detection of RNA targets for viral research and surveillance. 50X higher fidelity compared to Taq; Robust reactions - maximal success with minimal optimization; Master mix format with HF buffer ideal for a wide range of AT/GC content Home. Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. Contains 1 X 1 mL tube, sufficient for 100, 20 L reactions (10 L Master Mix per reaction). Fast SYBR Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for your real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency. Search. RT-LAMP master mix for faster and simpler detection of RNA targets for viral research and surveillance. PrimeTime Gene Expression Master Mix performs well under standard or fast cycling conditions (Figures 4 and 5) on a variety of real-time qPCR instruments, including the 7900HT Real-Time (Thermo Fisher Scientific), QuantStudio 7 Flex (Thermo Fisher Scientific), CFX384 (BioRad), and LightCycler 480 (Roche) qPCR systems. Get precise and reliable real-time qPCR quantification over nine logs of linear dynamic range with TaqMan Gene Expression Master Mix. Real Time PCR (qPCR) Technical Support Customer Service. Digital PCR products. Real-time PCR is the simultaneous amplification and detection/quantification of nucleic acids using PCR. Home. Real-time PCR is the simultaneous amplification and detection/quantification of nucleic acids using PCR. TaqPath qPCR Master Mix, CG: : 5 mL : TaqMan Fast Advanced Master Mix: : 5 mL Store at 28C. Shop All Products. Probe qPCR MixTaqManDual Labeled ProbePCRqPCRPCRPCRPCR Manufactured and quality-controlled at New England Biolabs, Thermo Scientific Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance. PrimeTime Gene Expression Master Mix performs well under standard or fast cycling conditions (Figures 4 and 5) on a variety of real-time qPCR instruments, including the 7900HT Real-Time (Thermo Fisher Scientific), QuantStudio 7 Flex (Thermo Fisher Scientific), CFX384 (BioRad), and LightCycler 480 (Roche) qPCR systems. (120 ng), the assay solution, and TaqMan Genotyping Master Mix (or another compatible master mix) (sold separately). PCR Equipment and Supplies. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance. Store at 28C. Get superior nucleic acid quantitation with Applied Biosystems Power SYBR Green Master Mix.Power SYBR Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations. Isothermal amplification. Search Thermo Fisher Scientific. Only template and primers Alternative Product: Try TaqPath ProAmp Master Mix, our newest and highest performing master mix for genotyping and copy number variation for accurate results, even from samples containing PCR inhibitors.With TaqPath ProAmp Master Mix, weve taken the best of TaqMan Genotyping Master Mix and added additional capabilities for your genotyping and copy number variation SuperScript IV VILO (SSIV VILO) Master Mix is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications. Real Time PCR (qPCR) Technical Support Customer Service. TaqMan Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target. Shop All Products. Search All . qPCR targets were quantitated with 50 ng 0.5 pg genomic DNA as input using an ABI 7500 Fast real-time instrument. qPCR targets were quantitated with 50 ng 0.5 pg genomic DNA as input using an ABI 7500 Fast real-time instrument. The Luna Universal Probe qPCR Master Mix is a 2X reaction mix optimized for real-time qPCR detection and quantitation of target DNA sequences using hydrolysis probes. The stability of this mix provides users of high-throughput, liquid handling systems the assurance that the results on the first plate will mimic those of the last plate. Real Time PCR (qPCR) Technical Support Customer Service. The 2x mix contains SYBR GreenER dye, AmpliTaq DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase, facilitating easy reaction set upjust add user-supplied assay and sample (Figure Home. Search All and instruments for qPCR. The Luna Universal Probe qPCR Master Mix is a 2X reaction mix optimized for real-time qPCR detection and quantitation of target DNA sequences using hydrolysis probes. TaqPath qPCR Master Mix, CG: : 5 mL : TaqMan Fast Advanced Master Mix: : 5 mL The TaqMan Fast Virus 1-Step Master Mix provides a single-tube format for uniform handling and processing, while delivering qPCR cycling in <30 minutes, helpful when working in high throughput workflows. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance. Shop All Products. The stability of this mix provides users of high-throughput, liquid handling systems the assurance that the results on the first plate will mimic those of the last plate. Use the real-time PCR master mix selection tool to find optimized master mixes, reagents, and kits to power your experiments. Search. Four fragments (~20 fmol) with 20 bp overlap were assembled using NEBuilder HiFi DNA Assembly Master Mix , GeneArt Gibson Assembly Mix (Thermo Fisher #A46627) and In-Fusion Snap Assembly Master Mix (Takara Bio USA #638947) to create a pUC19 vector. (120 ng), the assay solution, and TaqMan Genotyping Master Mix (or another compatible master mix) (sold separately). Search. Shop All Products. The 2x mix contains SYBR GreenER dye, AmpliTaq DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Four fragments (~20 fmol) with 20 bp overlap were assembled using NEBuilder HiFi DNA Assembly Master Mix , GeneArt Gibson Assembly Mix (Thermo Fisher #A46627) and In-Fusion Snap Assembly Master Mix (Takara Bio USA #638947) to create a pUC19 vector. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific. PCR Equipment and Supplies. Inclusion of ezDNase Enzyme further accelerates the RT-qPCR workflow through an extremely simplified genomic DNA removal step. TaqMan Fast Advanced Master Mix is designed to provide performance equal to or better than the results you currently expect from your standard master mix. Search Thermo Fisher Scientific. Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. Contains 1 tube (5 ml) 2X TaqMan Fast Advanced Master Mix sufficient for 500 reactions TaqMan Fast Advanced Master Mix contains AmpliTaq Fast DNA Polymerase, Uracil-N glycosylase (UNG), dNTPs (with dUTP), ROX dye (passive reference), and Genotyping & Genomic Profiling. Only template and primers In addition, TaqPath qPCR Master Mix, CG, has been engineered to retain consistent performance in preassembled reactions for up to 48 hours. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase, facilitating easy reaction set upjust add user-supplied assay and sample (Figure (This master mix is also available without ezDNase.) Search All . TaqPath qPCR Master Mix, CG: : 5 mL : TaqMan Fast Advanced Master Mix: : 5 mL Search Thermo Fisher Scientific. Search All . It provides best in class performance, including the widest quantifiable range, in both single and duplex reactions, even with challenging targets. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Home. Key product features: High sensitivity4X master mix to amplify both RNA and DNA with improved sensitivity Store at 28C. Only template and primers The Luna Universal Probe qPCR Master Mix is a 2X reaction mix optimized for real-time qPCR detection and quantitation of target DNA sequences using hydrolysis probes. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Reverse transcription . The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific. Invitrogen SuperScript enzymes and reagents for superior cDNA synthesis. qPCR is a versatile technique and a powerful tool to support your research applications. Search All . TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms. Figure 2: The Luna Universal qPCR Master Mix provides sensitive and accurate detection and quantitation across a wide variety of DNA sources The Luna Universal qPCR Master Mix is compatible with a broad range of genomic DNA sources. Reverse transcription . (This master mix is also available without ezDNase.) Get superior nucleic acid quantitation with Applied Biosystems Power SYBR Green Master Mix.Power SYBR Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations. The TaqMan Fast Virus 1-Step Master Mix provides a single-tube format for uniform handling and processing, while delivering qPCR cycling in <30 minutes, helpful when working in high throughput workflows. 50X higher fidelity compared to Taq; Robust reactions - maximal success with minimal optimization; Master mix format with HF buffer ideal for a wide range of AT/GC content SuperScript IV VILO (SSIV VILO) Master Mix is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications. Invitrogen SuperScript enzymes and reagents for superior cDNA synthesis. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase, facilitating easy reaction set upjust add user-supplied assay and sample (Figure 1 tube TaqMan assay mix 1 tube master mix 1 tube quantitative genomic DNA standard 1 bottle DNA dilution buffer 1 tube negative control water. TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms. Everything you need for SYBR Green dyebased PCR amplification and detection in a Everything you need for SYBR Green dyebased PCR amplification and detection in a TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms. (120 ng), the assay solution, and TaqMan Genotyping Master Mix (or another compatible master mix) (sold separately). Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. Search. Key product features: High sensitivity4X master mix to amplify both RNA and DNA with improved sensitivity Residual DNA Sample Preparation Kit contains: 1 box of buffers 1 box of magnetic beads 1 box of Proteinase K enzyme 1 box contains tRNA, Glycogen, Proteinase K Contains 1 tube (5 ml) 2X TaqMan Fast Advanced Master Mix sufficient for 500 reactions TaqMan Fast Advanced Master Mix contains AmpliTaq Fast DNA Polymerase, Uracil-N glycosylase (UNG), dNTPs (with dUTP), ROX dye (passive reference), and Search. Digital PCR products. Contains 1 tube (5 ml) 2X TaqMan Fast Advanced Master Mix sufficient for 500 reactions TaqMan Fast Advanced Master Mix contains AmpliTaq Fast DNA Polymerase, Uracil-N glycosylase (UNG), dNTPs (with dUTP), ROX dye (passive reference), and Fast SYBR Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for your real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency. TaqMan Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target. Search All and instruments for qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. Probe qPCR MixTaqManDual Labeled ProbePCRqPCRPCRPCRPCR Four fragments (~20 fmol) with 20 bp overlap were assembled using NEBuilder HiFi DNA Assembly Master Mix , GeneArt Gibson Assembly Mix (Thermo Fisher #A46627) and In-Fusion Snap Assembly Master Mix (Takara Bio USA #638947) to create a pUC19 vector. Inclusion of ezDNase Enzyme further accelerates the RT-qPCR workflow through an extremely simplified genomic DNA removal step. Contains 1 X 1 mL tube, sufficient for 100, 20 L reactions (10 L Master Mix per reaction). Home. Search Thermo Fisher Scientific. qPCR targets were quantitated with 50 ng 0.5 pg genomic DNA as input using an ABI 7500 Fast real-time instrument. Use the real-time PCR master mix selection tool to find optimized master mixes, reagents, and kits to power your experiments.
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